Determining Plasma Protein Binding for High Binders Using Equilibrium Gel Filtration with Non- radiolabeled Compounds
نویسندگان
چکیده
منابع مشابه
INVESTIGATIONS ON THE DRUG-PROTEIN IN TERAC TION OF CERTAIN NEW POTENTIAL LOCAL ANAESTHETICS
Generally, plasma proteins owe their binding capacity to the presence of aminoacid units which enter into intra- and intermolecular hydrophobic bonding with a diverse range of endo- and exogenous chemical substances. The intermolecular interactions between the hydrophobic areas of drug molecules and those of plasma proteins play an important role in drug-macromolecular complex formation and...
متن کاملDetermination of transmembrane pH gradients and membrane potentials in liposomes.
Techniques for determining large transbilayer pH gradients (delta pH) and membrane potentials (delta psi) induced in response to delta pH in large unilamellar vesicle liposomal systems by measuring the transbilayer redistribution of radiolabeled compounds have been examined. For liposomes with acidic interiors, it is shown that protocols using radiolabeled methylamine in conjunction with gel fi...
متن کاملCharacterization of drug-plasma protein interactions using surface plasmon resonance
Binding to plasma proteins is a key parameter in evaluating candidate compounds during the lead optimization and early ADME phases of the drug development process. Biacore’s surface plasmon resonance (SPR) technology is ideally suited for the analysis of multiple aspects of drug-plasma protein interactions. BiacoreS51 was used to analyze a panel of low molecular weight drug compounds for bindin...
متن کاملCharacterization of drug-plasma protein interactions using surface plasmon resonance
Binding to plasma proteins is a key parameter in evaluating candidate compounds during the lead optimization and early ADME phases of the drug development process. Surface plasmon resonance (SPR) technology is ideally suited for the analysis of multiple aspects of drug-plasma protein interactions. Here we describe how a BiacoreTM SPR based system was used to analyze a panel of low molecular wei...
متن کاملtudy on sex steroid-binding proteins (with emphasize on 17 -estradiol) in plasma of female and juvenile kutum (Rutilus frisii kutum)
A sex steroid-binding protein (SBP) that binds to 17 b-estradiol with high affinity and moderate capacity was identified in the plasma of female and juvenile of kutum (Rutilus frisii kutum) sampled during the early stage of gonadal maturation in October and prior to spawning in March. Mean maximum specific binding (Bmax) and equilibrium dissociation constant (Kd) of the fish were as follows: In...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016